Journal: Nature Communications

Article Title: Cardiac radiotherapy induces electrical conduction reprogramming in the absence of transmural fibrosis

doi: 10.1038/s41467-021-25730-0

Figure Lengend Snippet: a Cardiac 17-segment model illustrating 25 Gy targeted (Segment 1, red) and remote nontargeted (Segment 10, gray) myocardium collected from a heart failure patient with nonischemic cardiomyopathy and VT (Patient F), who previously received cardiac RT. The nontargeted Segment 10 received <5 Gy radiation exposure. For comparison, corresponding Segments 1 and 10 were collected from 2 nonfailing, non-RT donor hearts rejected for transplantation. b Western blot and quantified densitometry of Na V 1.5 in Segment 10 versus Segment 1 of nonfailing (Donors A and B) and RT-treated (HF+Cardiac RT, red) hearts. c Western blot and quantified densitometry of Cx43 in Segment 10 versus Segment 1 of nonfailing and RT-treated hearts. d Matched QRS durations in the ENCORE-VT patient cohort at baseline (pre-RT, 149 ± 34 ms) versus 6-week post-RT (139 ± 32 ms) timepoints ( n = 19 paired individual patients, P = 0.072 by two-tailed Wilcoxon signed-rank test). e Representative 12-lead ECG of robust QRS shortening recorded from Patient G at the time of RT treatment, with a baseline QRS of 165 ms and presence of LBBB, and then after 6 months post-RT, exhibiting shortened QRS (130 ms) and resolution of LBBB. Examples of pre- and post-RT ECGs of the remaining three patients that exhibited robust QRS shortening in the ENCORE-VT patient cohort, as well as an example of an unchanged QRS interval, are provided in the Supplementary Figures. Source data are provided as a Source data file.

Article Snippet: Lead II ECGs were recorded at a sampling rate of 4 kHz using LabChart (Version 8.0, ADInstruments), and ECG intervals of 100 averaged beats were measured using the LabChart ECG software package .

Techniques: Comparison, Transplantation Assay, Western Blot, Two Tailed Test

Journal: Frontiers in Physiology

Article Title: Noninvasive Assessment of the Effect of Position and Exercise on Pulse Arrival to Peripheral Vascular Beds in Healthy Volunteers

doi: 10.3389/fphys.2017.00047

Figure Lengend Snippet: Example reading of LabChart file . We simultaneously recorded the ECG (bottom) along with plethysmographs from big toe (first from the top), finger (second from the top), and ear (third from the top) and for 30 s each in standing, sitting, and supine positions. The PAT to each location (ear lobe, index finger, and big toe) was assessed by calculating the time delay between the peak of the R wave on the ECG and the first subsequent positive inflection on the plethysmograph trace. Periods consisting of 10 consecutive heart beats from each position (standing, sitting, supine) before and after exercise were then used to calculated the mean PAT for the three locations (ear lobe, index finger, big toe). ECG, electrocardiogram; PAT, pulse arrival time.

Article Snippet: The ECG and plethysmograph signals were simultaneously digitally converted at 1 kHz (PowerLab 4/26, ADInsruments, Australia) and analyzed by eye and hand (LabChart 8.0, ADInstruments, Australia).

Techniques: